EVALUATION OF SOME CHEMICAL CONSTITUENTS OF SELECTED ENERGY DRINKS
This research work examined and compares the physicochemical properties and some chemical constituents of selected energy drinks. Fourteen (14) brands of energy drinks samples consisting eleven (11) liquid and three (3) powdered forms were randomly purchased. All samples were analyzed for their physicochemical properties (pH, turbidity, conductivity and total dissolved solids), trace and heavy metals, aspartame, sugar and caffeine contents. Results showed that the physicochemical properties (i.e. pH, turbidity, conductivity and total dissolved solids) ranged from 4.47 ± 0.012 – 5.96 ± 0.012, 8 ± 0.577 – 592 ± 1.155 NTU, 2.21 ± 0.006 – 1975 ± 1.732 µs/cm, and 243 ± 0.577 – 1064 ± 0.577 mg/L respectively. Energy drinks analyzed all fell within the FDA recommended range for the physicochemical properties analyzed. Iron, calcium, zinc and potassium were found in all the energy drinks and their concentration ranged from 1.961 ± 0.0003 – 0.294 ± 0.0005 mg/L, 2.763 ± 0.0009 – 19.310 ± 0.0015 mg/L, 0.045 ± 0.0001 – 13.887 ± 0.0037 mg/L, and 2.0 to 2500 mg/L respectively. The copper, lead and manganese concentration of energy drinks ranged from 0.002 ± 0.0002 – 0.102 ± 0.0003 mg/L, 0.028 ± 0.0006 – 0.209 ± 0.0009 mg/L and 0.003 ± 0.0001 – 0.024 ± 0.0002 mg/L respectively. The concentration of copper and manganese were below the MCL of 1.0 mg/L and 0.05 mg/L respectively while lead had a concentration above the MCL of 0.01 mg/L. Cadmium was not detected in all energy drinks except for sample EJ which had a concentration of 0.102 ± 0.0003 mg/L and exceeded the MCL of 0.005 mg/L. The caffeine, aspartame and sugar concentrations ranged from 1.11 mg/L – 2487.13 mg/L, 6.51 mg/L – 1491.19 mg/L, and 16.98 – 1686.73 mg/L respectively. Caffeine and aspartame concentrations in all the energy drink samples were below the FDA set standard of 400 mg/L and 3000 mg/L respectively except for sample AL which had a concentration above the set standard for caffeine. Though the analyzed parameters were mostly below the set standards, especially caffeine, aspartame and sugar, it is important that the pattern of consumption of these drinks must be monitored to minimize ingestion of excess doses of harmful substances to prevent the reported adverse effects.
TABLE OF CONTENTS
Table of Contents
List of Abbreviations
1.1 Energy Drinks
1.2 Contents of Energy Drinks
1.4 Aim and Objectives
2.0 LITERATURE REVIEW
2.1 Review Work on Energy Drinks
2.2.1 Metabolism of caffeine
2.2.2 Caffeine and health
2.2.3 Research works on caffeine
2.3.1 Chemistry of aspartame
2.3.2 Biochemical data
2.4 Heavy Metal
2.5.2 Added sugars
2.5.3 Sugar-sweetened beverage
2.5.4 Sugar-sweetened beverages and health risks
2.6 Research Work on Beverages
3.0 MATERIALS AND METHODS
3.1.1 Chemicals and reagents
3.1.2 Preparation of stock solution
220.127.116.11 Preparation of standard solution for AAS
18.104.22.168 Preparation of standard solution for HPLC
22.214.171.124 Preparation of standard solution for UV
3.1.3 Apparatus and equipments
3.1.4 Sample collection
3.1.5 Sample preparation
126.96.36.199 Sample preparation for AAS
188.8.131.52 Sample preparation for HPLC
184.108.40.206 Sample preparation for UV
3.2 Analysis of Physicochemical Properties
3.2.1 Determination of pH
3.2.2 Determination of conductivity
3.2.3 Determination of turbidity
3.2.4 Total dissolved solid
3.3 Elemental Analysis
3.4 Determination of Carbohydrates (Sugar)
3.5 Determination of Caffeine and Aspartame
3.5.1 Preparation of pH 4.0 and pH 7.0 buffer solution
3.5.2 Buffer preparation
3.5.3 Mobile phase preparation
3.6 Statistical Analysis
4.1 Physicochemical Parameters of Samples
4.2 Metal Concentrations
4.2.1 Concentration of heavy metals
4.2.2 Concentrations of essential metals
4.3 Concentrations of Caffeine, Aspartame and Sugar
5.1 Physicochemical Parameters of the Samples
5.1.3 Total dissolved solids
5.2 Metal Concentrations
5.2.1 Heavy metals
5.2.2 Essential Metals
5.3 Caffeine, Aspartame and Sugar Concentrations in Energy Drinks
List of Abbreviations
AAS Atomic Absorption Spectroscopy
ATP Adenosine Triphosphate
CYP1A2 Cytochrome P450 1A2
CYP2A6 Cytochrome P450 2A6
DKP 5-benzyl-3, 6-dioxo-2-piperazine acetic acid
DNA Deoxyribonucleic Acid
EU European Union
FAO Food and Agriculture Organization
FDA Food and Drinks Administration
GC–MS Gas Chromatography–Mass Spectrometry
HDL High Density Lipoprotein Cholesterol
HPLC High Performance Liquid Chromatographic
NAT2 N-acetyltransferase 2
NNS National Nutrition Survey
RDA Recommended Daily Allowance
RNA Ribonucleic Acid
SCF Scientific Committee for Food
SSB Sugar Sweetened Beverages
USA United States of America
UV Ultraviolet Visible Spectroscopy
WHO World Health Organization
Energy drinks refer to beverages that contain large doses of caffeine and other legal stimulants such as taurine, carbohydrates, glucuronolactone, inositol, niacin, panthenol, and β-complex vitamins which are considered as source of energy (Attila and Çakir, 2009). The consumption of readily available energy drinks has increased significantly with young adults forming the largest part of the consumers. History of energy drink dates back to 1987 when Red Bull was introduced in Austria. It became more popular in the 1990s following its introduction to the United States. Since then the sale of this drink has increased exponentially. In 2006, the energy drink market grew by 80% (Foran et al., 2011). This is because manufactures claim the drinks can boost energy levels as well as physical endurance, improve concentration and reaction speed (Van den Eynde et al., 2008).
In recent years, a number of different energy drinks have been introduced in the Nigerian market to provide an energy boost or as dietary supplements. These drinks are marketed specifically to children and young adults. These products have been used for various reasons. A survey conducted among college students shows that 67% of students admitted using energy drinks to cope with insufficient sleep, 65% mentioned increasing energy and 54% use it for fun at parties; 50% for studying or completing a major course project, 45% used it while driving a car for a long period of time and 17% for treating hangover (Malinauskas et al., 2007). These products have also been used to reduce the depressor effect of alcohol or even to gain social status (Ferreira et al., 2004; Kaminer, 2010).
Many energy drinks are promoted as being nutraceutical foods, boosting health, energy, or otherwise having sought-after benefits. There is some concern among health professionals that these beverages, and the drinking behaviours of the targeted consumers, may in fact have adverse health consequences. The most commonly reported adverse effects include insomnia, nervousness, headache, and tachycardia (Clauson et al., 2008). In a recent study, heavy consumption of energy drinks was attributed to new onset seizures in four patients (Iyadurai and Chung, 2007) and hospitalization of individuals with pre-existing mental illness (Chelben et al., 2008).
- Energy Drinks
Energy drinks first appeared in Europe and Asia in the 1960s in response to consumer demand for a dietary supplement that would result in increased energy (Reissig et al., 2008). In 1962, a Japanese company, Taisho Pharmaceuticals, launched Lipovitan D, one of the very first energy drinks, which is still dominating the Japanese market. Since the 1960s, the energy drink market has grown into a multibillion dollar business which has been reported as being the fastest growing segment in the beverage industry. Energy drinks have established a viable position in the beverage market as evidenced by their commonplace consumption in the morning, afternoon, and night, not only by the general consumer, but those of age 18 to 34 in particular (Lal, 2007).
The popularity of energy drinks and the growth in their consumption among adolescents and young adults have brought worries regarding general health and well being of these consumers. Adolescents and young adults are often uninformed about the content of energy drink (Rath, 2012).
- Contents of Energy Drinks
There are hundreds of energy drinks available in the market, many share very similar ingredient profiles. Most of these energy drinks consist mainly of caffeine, Taurine, Guarana, Ginseng, B vitamins, Ginkgo Biloba, L-carnitine, sugars, Antioxidants, Glucuronolactone, Yerba Mate, Creatine, Acai Berry, Milk Thistle, L-theanine, Inositol and artificial sweeteners (Babu et al., 2008).
Caffeine is probably the most frequently ingested pharmacologically active substance in the world. It is one of the main ingredients of stimulant drinks and it is also present in tea, coffee and other beverages and foods. Caffeine is extracted from the raw fruit of over sixty species of coffee plants (coffea Arabica), all part of the methylxanthine family. The dimethyl xanthine derivatives, theophylline and theobromine, are also found in a variety of plants. It is also extracted from tea, kola nuts, and cocoa. The average total intake of caffeine in the Republic of Ireland and the UK is estimated to be 214 and 278 mg per person per day, respectively (FSPB, 2010). Data from the consumption survey, based on weekly intake, indicates that among stimulant drink consumers, the average daily caffeine intake from stimulant drinks alone would be approximately 35 mg, rising to about 90 mg among the highest consumers (FSPB, 2010). This does not appear excessive. However, when the consumption of stimulant drinks in a single session was investigated, the average caffeine consumed was approximately 240 mg (3 cans), rising to about 640 mg (8 cans) among the highest consumers (FSPB, 2010). Such large intake levels among the highest consumers are a cause of concern, particularly in relation to the known potential acute health effects of caffeine such as tachycardia, increases in blood pressure and dehydration, as well as behavioural and cognitive effects. The health effects of chronic or habitual caffeine consumption remain uncertain.
Taurine (2-aminoethanesulfonic acid) is a sulfur containing amino acid that is the most abundant amino acid found naturally in our bodies, primarily in the retina and skeletal and cardiac muscle tissue (Timbrell et al., 1995; Imagawa et al., 2009). Taurine is derived from the metabolism of methionine and cysteine (Huxtable, 1992; Stipanuk, 2004). It is also present in common food items such as meat and fish. The data on stimulant drink intake among stimulant drink consumers indicate that average daily taurine intake from stimulant drinks was approximately 0.4 g, increasing to about 1 g among the highest consumers (FSPB, 2010). The most taurine consumed from stimulant drinks in a single session was averaged at approximately 3 g, rising to about 8 g by the highest consumers (FSPB, 2010). Stimulant drink intake at the maximum level of intake provides taurine far in excess of that from other foods or beverages in the diet. Data available indicate no evidence of adverse effects of taurine at such intakes and in a recent report the EU Scientific Committee for Food (SCF) was unable to conclude that the ‗safety-in-use‘ of taurine in the concentration range reported for stimulant drinks has been adequately established (EU SCF, 1983).
Guarana (Paullinia cupana) is a native South American plant containing guarana, a substance chemically similar to caffeine with comparable stimulant effects. Guarana is often added to stimulant drinks, either in combination with caffeine or on its own. The stimulant effect of guarana is related to its caffeine content; one gram of guarana contains as much caffeine (40 mg) as a medium strength cup of coffee. The precise source and nature of the stimulant activity of guarana is not well understood. However, it has been reported that guarana exerts a more prolonged effect than an equivalent amount of caffeine. The Food and Drinks Administration (FDA) in the USA currently prohibits the use of guarana in food and drinks while awaiting further clarification on its safety (USFDA, 2011).
This is a naturally occurring substance produced in small amounts within the body. Supplementation with d-glucarates, including glucuronolactone, may favor the body‘s natural defense mechanism for eliminating carcinogens and tumor promoters and their effects (Zołtaszek et al., 2008). The data from the consumption survey indicate that average daily glucuronolactone intake from stimulant drinks was approximately 0.25 g, rising to about 0.7 g among the highest consumers (FSPB, 2010). The most glucuronolactone consumed from stimulant drinks in a single session was averaged at approximately 1.8 g, rising to about 4.8 g among the highest consumers (FSPB, 2010). These maximum levels of intake provide more glucuronolactone than would otherwise be achieved through other foods or beverages in the diet. There is very little information available for risk assessment of glucuronolactone at such intakes. There is no indication from the available data that there is any risk to health from consumption of high amounts of glucuronolactone, although these data are limited.
Ginseng is a herb that has been used for over 2000 years by people in East Asian countries including China, Japan, and Korea as a remedy for various diseases and for promoting longevity (Lee et al., 2005; Nam et al., 2005). Panax ginseng is the primary commercial species and is often referred to as Korean or Asian ginseng. Siberian ginseng (Eleutherococcus senticosus) is not truly a ginseng since it contains eleutherosides as its active constituent and no ginsenosides. P. ginseng is a small, shade loving perennial shrub that reaches about 60cm in height and belongs to the plant family Araliacae. The entire ginseng plant has been used for medicinal purposes; however, the root is the most prominent and dominates the commercial sales. Ginseng has been incorporated into a variety of energy drinks although little medical literature supports these uses. Adverse effects associated with ginseng use tend to be mild. However more serious complications have been reported, including diarrhoea, vaginal bleeding, severe headache, and Stevens-Johnson syndrome (Enerst, 2002; Dega et al., 1996). Many of these effects may be attributed to contaminants. Agranulocytosis in 4 patients taking ginseng had been linked to unreported phenylbutazone and aminopyrine contained in the preparation (Ries et al.,1975). A ginseng abuse syndrome, characterized by morning diarrhoea, hypertension, rashes, insomnia, and irritability had been reported (Siegel, 1979). Little is known regarding the effects of ginseng in children and adolescents (Braganza and Larkin, 2007).
B vitamins are a group of 8 individual water-soluble vitamins, usually referred to as the B complex when grouped together, and all play essential roles in cellular processes. B vitamins are incorporated into many of the mainstream energy drinks. A typical can of 250ml may contain 360% of the recommended daily allowance (RDA) of B6, 120% of B12, and 120% of B3 (niacin). The container size varies among brands and it may hold multiple servings. The addition of excess amounts of B vitamins is also observed in the more extreme energy drinks like 5-Hour Energy which contains 8333% of the RDA for vitamin B12 and 2000% of the RDA for B6. It is claimed that the consumption of these large amounts of B vitamins increases mental alertness and focus, as well as improves mood. The average person, however, consumes the RDA of B vitamins from a typical diet since B vitamins are found in a variety of foods including bananas, lentils, potatoes, tuna, and turkey. Vitamins B2 (riboflavin), B3 (niacin), B6 (pyridoxine, pyridoxal, pyridoxamine), and B12 are the most common of the B vitamins that are incorporated into energy drink formulations (Wardlaw and Smith, 2009).
Vitamin B2 is a coenzyme in the metabolism of carbohydrates. Vitamin B3 plays a major role as a coenzyme in energy metabolism, fat synthesis, and fat breakdown (Wardlaw and Smith, 2009). Vitamin B6 is a group of 3 structurally similar compounds that all can be converted into the vitamin B6 coenzyme which aids in the utilization of carbohydrates, fats, and proteins (Wardlaw and Smith, 2009). Vitamin B12 assists in folate metabolism and in nerve function (Wardlaw and Smith, 2009). Since all of the B vitamins are water soluble, once the RDA has been met, the excess vitamins are excreted from the body via urine. Although the consumption of a large amount of B vitamins does not possess any adverse health effects, the logic behind the extreme amounts of B vitamins in these beverages is not well rationalized (Wardlaw and Smith, 2009).
Sugar is one of the most common as well as most dangerous additives on the market today.
It‘s sweet taste and short-term positive effects lead health experts to consider the possibility of sugar addiction. Natural sugars, such as those found in fruits and dairy products, provide an easily digestible form of good-for-you energy. The synthetic copycats like refined sugar, sucrose, fructose and glucose do far more harm than good (Nash, 1992).
More than 32 g of extra sugar a day can cause a myriad of health problems (Bauer, 2011) such as raised cholesterol levels, suppressed immune system, hyperactivity, anxiety, difficulty concentrating, crankiness, decreased emotional stability, a raised level of neurotransmitters, hypoglycemia, increased blood pressure, interference with protein absorption, and impaired DNA structure (Appleton, 2011). Just one serving of Red Bull contains 27 g of sugar (Red Bull Energy drink USA), and the average cup of coffee contains 32 g (Starbucks coffee company) (Nash, 1992).
Energy drinks have established an enviable position in the beverage market as evidenced by their commonplace consumption. There are a number of scientific reports on the adverse consequences of excessive consumption of these drinks. Many of these products do not provide the complete chemical composition, and the caffeine content and other ingredients present are unknown to the consumer. Hence there is need to quantify the major content of these energy drinks and compare with those of accepted standards. Also, energy drinks occur mostly in liquid and powdered forms. There has been little or no research on the powdered products as more attention has been given to those in liquids.
These powdered products are usually dissolved in water by consumers before intake. It is therefore imperative to determine the caffeine, aspartame and other energizers of the powdered products and compared them with those of the liquid products. It will also be necessary to determine some other physicochemical properties of the energy drinks.
- Aim and Objectives
The aim of this work is to carry out comparative study on the physicochemical properties and some chemical constituents of selected energy drinks. This will be achieved through:
- determination of the caffeine and aspartame concentrations of the energy drinks;
- determination of the carbohydrate (sugar) contents;
- determine the physicochemical properties of energy drinks;
- determination of the level of heavy metals (Cu, Zn, Pb, Cd, Mn, Fe) in them;
- determination of macronutrients (K, Ca,) present in them;
- using Statistical Analysis to analyse and compare between the powdered and liquid forms of energy drinks;
- comparing the obtained results with set standards by regulatory bodies.
Acheson, K. J., Zahorska-Markiewics, B., Pittet, P., Anantharraman, K., Jequier, E. (1980). Caffeine and coffee: their influence on metabolic rate and substrate oxidation in normal weight and obese individuals. Am J Clin Nutr 33: 989–97.
Adeleke, R. O., Abiodun, O. A. ( 2010). Physico-chemical Properties of Commercial Local Beverages in Osun State, Nigeria. Pakistan Journal of Nutrition 9 (9): 853-855.
Alghamdi, A. H., Alghamdi, A. F. and Alwarthan, A. A. (2005). Determination of Content Levels of Some Food Additives in Beverages Consumed in Riyadh City. J. King Saud Univ., 18(2): 99-109.
Al-Mayaly K. I. (2013). Determination of heavy Metals in some Artificial Fruit juices in Iraqi Local Markets. Intern. Journal. Of Research and Dev. In Pharm. And Life Sci. 2(4): 507-510.
Appleton, N. “146 Reasons Why Sugar Is Ruining Your Health.” Antibiotics to Treat. November 15, 2011.
Aschner, M., (2000). Manganese: brain transport and emerging research needs. Environ. Health Perspect. 108 (Suppl. 3), 429–432.
Ashurst, P.R., 2005. Chemistry and Technology of Soft drinks and Fruit Juices. 2nd Edn., Vol. III (4) Blackwell Scientific Publication, London, pp: 433.
Astrup, A., Dyerberg, J., Selleck, M. and Stender, S. (2008). Nutrition transition and its relationship to the development of obesity and related chronic diseases. Obesity review. 9: 48-52.
Astrup, A., Toubro, S., Cannon, S., Hein, P., Breum, L., Madsen, J. (1990). Caffeine: a doubleblind, placebo-controlled study of its thermogenic, metabolic, and cardiovascular effects in healthy volunteers. Am J Clin Nutr 51: 759–67.
Attila, S., and Çakir, B. (2011). Energy-drink consumption in college students and associated factors. Nutrition, 27(3): 316-322.
Attieh, Z. K., Mukhopadhyay, C. K., Seshadri, V., Tripoulas, N. A., Paul, L. (1999). FoxCeruloplasmin Ferroxidase Activity Stimulates Cellular Iron Uptake by a Trivalent Cation-specific Transport Mechanism, J. Biol Chem., 274: 1119.
Ayala, J., Simons, K., and Kerrigan, S. (2009). Quantitative determination of Caffeine and Alcohol in energy drinks and the potential to produce positive transdermal alcohol concentrations in human subjects. Journal of analytical Toxicology. Vol. 33
Babu, M., Kavita, M. D., Church, J., Richard, M. D., Lewander, William, M. D. (2008). Energy Drinks: The New Eye-Opener for Adolescents. Clinical Paediatric Emergency Medicine, 9(1): 35-40.
Barbaste, M., Medina, B., Perez-Trujillo, J. P. (2003). Analysis of arsenic lead and cadmium in wines from the Canary Islands, Spain, by ICP/MS. Food Additives and Contaminant, 20: 141–148.
Barbee, Jr. J. Y., Prince, T. S. (1999). Acute respiratory distress syndrome in a welder exposed to metal fumes. South Med J; 92: 510–2
Bassiouny, M. A. and Yang, J. (2005). Influence of drinking patterns of Carbonated beverages on dental erosion. General Dentistry, 53: 207-210.
Bauer, J. (2011). “Sugar: How Much Should We Eat?” Yahoo! Health. Web. 08 Dec.
Bengol, M., Yentur, G., Er B., Oktem, A.,B. (2010). Determination of some heavy metal levels in soft drinks from Turkey using ICP/OES method. Czech J. Food Sci., 28: 213–216.
Berkey, C. S., Rockett, H. R., Field, A. E., Gillman, M. W. and Colditz, G. A. (2004). Sugar added beverages and adolescent weight change. Obesity research. 12(5): 778-88.
Bonnet, M. H., Balkin, T. J., Dinges, D. F., Roehrs, T., Rogers, N. L., Wesensten, N. J. (2005). The use of stimulants to modify performance during sleep loss: a review by the Sleep Deprivation and Stimulant Task Force of the American Academy of Sleep Medicine. Sleep 28: 1163–87.
Bracco, D., Ferrarra, J. M., Arnaud, M. J., Jequier, E., Schutz, Y. (1995). Effects of caffeine on energy metabolism, heart rate, and methylxanthine metabolism in lean and obese women. Am J Physiol Endocrinol Metab 269: 671–78.
Braganza, S. and Larkin, M. (2007). Riding high on energy drinks. Modern medicine. Available at: http://www.modernmedicine.com/modernmedicine/ article/articleDetail.jsp?id=426763. Posted May 15, 2007. [Accessed September 15, 2007].
Brand-Miller, J. C., Holt, S. H., Pawlak, D. B. and McMillan, J. (2002). Glycemic index and obesity. American Journal of Clinical Nutrition. 76(1): 281S-5S.
Brownell K. D. and Frieden T. R. (2009). Ounces of prevention—the public policy case for taxes on sugared beverages. N Engl J Med; 360:1805-8.
Brownell, K. D., Farley, T., Willett, W. C., (2009). The public health and economic benefits of taxing sugar sweetened beverages. N Engl J Med.; 361:1599-605.
Butchko, H. H., and Stargel, W. W. (2001). Aspartame: Scientific evaluation in the postmarketing period. Regulatory Toxicology and Pharmacology, 34:221–233.
Cabrera, C., Lorenzo, M. L., Lopez, M. C. (1995). Lead and cadmium contamination in dairy products and its repercussion on total dietary intake. Journal of Agricultural and Food Chemistry, 43: 1605–1609.
Carrillo, J.A., and Benitez, J. 2000. Clinically significant pharmacokinetic interactions between dietary caffeine and medications. Clin. Pharmacokinet, 39:127–153.
Chanarin, I. (1999). Nutritional aspects of hematological disorders. In: Shils, M.E., Olson, J.A., Shike, M., Ross, A.C. (Eds.), Modern Nutrition in Health and Disease. Lippincot, Williams, & Wilkins, Baltimore, pp. 1419–1436.
Chelben, J., Piccone-Sapir, A., Ianco, I., Shoenfeld, N., Kotler, M. and Strous, R. D. (2008). Effects of amino acid energy drinks leading to hospitalization in individuals with mental illness. Gen. Hosp. Psychiatry, 30(2): 187–189.
Choi, H. K, Willett, W. W., Curhan G. (2010). Fructose-rich beverages and risk of gout in women. JAMA, 304.20:2270 – 2278.
Choi, H. K. and Curhan, G. (2008). Soft drinks, fructose consumption, and the risk of gout in men; prospective cohort study. BMJ, 336:309 – 312.
Clauson K. A., Shields K. M., McQueen C. E., and Persad N. (2008). Safety issues associated with commercially available energy drinks. J. Am. Pharm. Assoc. 48(3): 55–63.
Coulombe, R. A., Sharma, R. P. (1986). Neurobiochemical alterations induced by the artificial sweetener aspartame (NutraSweet). Toxicol Appl Pharm, 83(1): 79-85.
Craig, T. D. C. (2010). soda tax proposal draws opposition from beverage industry.
Washington Post. May 14, 2010.
Crews, H.M., Olivier, L., andWilson, L.A. 2001. Urinary biomarkers for assessing dietary exposure to caffeine. Food Addit. Contam., 18: 1075–1087.
Crowe, T. C., Fontaine, H. L., Gibbon, C. J., Cameron-Smith, D. and Swinburn, B. A. (2004). Energy density of food and beverages in the Australian food supply: influence of macronutrients and comparison to dietary intake. European Journal of Clinical Nutrition. 58(11): 1485-91.
Cysneiros, R. M., Farkas D., Harmatz J. S., von Moltke L. L., Greenblatt D. J. (2007). Pharmacokinetic and pharmacodynamic interactions between zolpidem and caffeine. Clin Pharmacol Ther 82: 54–62.
Dailey, J. W., Lasley, S.M., Burger, R. L., Bettendorf, A. F., Mishra, P. K. and Jobe, P. C. (1991). Amino acids, monoamines and audiogenic seizures in genetically epilepsy-prone rats: effects of aspartame. Epilepsy Res. 8 122–133.
Das, A. (1990). Metal ion induced toxicity and detoxification by chelation therapy. In:1stA textbook on medical aspects of bioinorganic chemistry, CBS, Delhi, P. 17-58.
Davis, C. D. and Greger, J. L. (1992). Longitudinal changes of manganese-dependent superoxide dismutase and other indexes of manganese and iron status in women.
Am. J. Clin. Nutr. 55, 747–752.
Dega, H., Laporte, J. L., and Frances, C. (1996). Ginseng as a cause for Stevens- Johnson syndrome. Lancet, 347:1344.
Devine, A. (1999). A Four Year follow-up Study of the Effects of Calcium Supplementation on Bone Density in Elderly post-Menopausal Women. Osteoporos Int. 7: 23-28.
Dhingra, R., Sullivan, L., Jacques, P. F., Wang, T. J., Fox, C. S., Meigs, J. B., D’Agostino, R. B., Gaziano, J. M., and Vasan, R. S. (2007). Soft drink consumption and risk of developing cardiometabolic risk factors and the metabolic syndrome in middle-aged adults in the community. Circulation, 116.5:480 – 488.
Diomede, L., Romano, M., Guiso, G., Caccia, S., Nava, S. and Salmona, M. (1991). Interspecies and interstrain studies on the increased susceptibility to metrazol-induced convulsions in animals given aspartame. Food Chem. Toxicol. 29 101– 106.
Doherty, M. and Smith, P. M. (2004). Effects of caffeine ingestion on exercise testing: a meta-analysis. Int J Sport Nutr Exerc Metab 14: 626–46.
Drake, I., Sonestedt, E., Gullberg, B., Ahlgren, G., Bjartell, A., Wallström, P., Wirfält, E. (2012). Dietary intakes of carbohydrates in relation to prostate cancer risk: a prospective study in the Malmö Diet and Cancer cohort. Am J Clin Nutr, 96, 1409 – 1418.
Duffus, J. H. (2002). ―Heavy metal‖ – a meaningless term? Pure Appl Chem 74:793–807.
Dulloo, A. G., Geissler, C. A., Horton, T., Collins, A., Miller, D. S. (1989). Normal caffeine consumption: influence on thermogenesis and daily energy expenditure in lean and post-obese human volunteers. Am J Clin Nutr 49: 44–50.
Dunwiddie, T. V., and Mansino, S. A., (2001). The role and regulation of adenosine in the central nervous system. Ann Rev Neurosci, 24: 31–55.
Ebbeling, C, Feldman, H, Osganian, S, (2006). ―Effects of Decreasing Sugar-sweetened Beverage Consumption on Body Weight in Adolescents: A Randomized, Controlled Pilot Study.‖ Pediatrics, 117(3): 673–680.
(EFSA) European Food Safety Authority (2010). Scientific Opinion on Dietary Reference Values for carbohydrates and dietary fibre. EFSA Journal. 8(3):1462.
Enerst, E. (2002). The risk-benefit profile of commonly used herbal therapies: Ginkgo, St. John’s Wort, Ginseng, Echinacea, Saw Palmetto, and Kava. Ann Intern Med 136:42-53.
EU SCF (European Union Scientific Committee for Food) (1983). Report of the Scientific Committee for Food on Caffeine. Reports of the Scientific Committee for Food (Fourteenth Series). Commission of the European Communities, Luxembourg. Vol.15(1-3)
FAO/WHO, (Food and Agriculture Organization/World Health Organization). (1998). Carbohydrates in Human Nutrition. Rome: FAO. vol. 165.
FAO/WHO, (1980). Toxicological evaluation of certain food additives: Aspartame. WHO Food Additive Series No. 15. The Joint FAO/WHO Expert Committee on Food Additives, Geneva, Switzerland, pp: 18-86.
FDA, (Food and Drug Administration) (1996). Inactive Ingredients Database. Aspartame. US Food and Drug Administration. Center for Drug Evaluation and Research (CDER). http://www.accessdata.fda.gov/scripts/cder/iig/index.cfm. (site visited on January 16, 2006).
FDA, (Food and Drug Administration) (2003). Substances Generally Recognized as Safe. Code of Federal Regulations. Title 21, vol.3. Sec.182.1180. Consulted on 14.03.2012 available at http://coffeefaq.com/site/node/13. Food Chem Toxicol.; 40(9): 1271–1310.
Fernstrom, J. D., Fernstrom, M. H. and Gillis, M. A. (1983). Acute effects of aspartame on large neutral amino acids and monoamines in rat brain. Life Sci. 32 1651–1658
Ferre, S. (2008). An update in the mechanisms of the psychostimulant effects of caffeine. J Neurochem, 105:1067–79.
Ferreira, E. S., Hartmann, Quadrosi, M. I., Trindade, A. A., Takahashi, S., Koyama, G. R., Souza-Formigoni, O. M. (2004). Can energy drinks reduce the depressor effect of ethanol? An experimental study in mice. Physiology and Behaviour, 82(5): 841-847.
Finley, J. N. (2004). Does environmental exposure to Manganese pose a health risk to healthy adults? Nutr. Rev., 62, 148-153.
Foran, M., Strickland, F., Perkins, K., Smith, J. A. (2011). Excessive Intraoperative Bleeding With Chronic Energy Drink Consumption. Journal of Oral and Maxillofacial Surgery, 1-3.
Fotherby, M. D. and Potter J. F. (1992). Potassium Supplementation Reduces Clinic and Ambulatory Blood Pressure in Elderly Hypertensive Patients. J Hypertension. 10: 1403-8.
Fraga, C. G. and Oteiza, P. I. (2002). Iron toxicity and antioxidant nutrients. Toxicology 180, 23–32.
FSPB (Food Safety Production Board) (2010). A review of Health Effects of Stimulated Drinks. Final report. Republic of Ireland. Pp 1-40
Genkinger, J. M., Li, R., Spiegelman, D. (2012). Coffee, tea, and sugar-sweetened carbonated soft drink intake and pancreatic cancer risk: a pooled analysis of 14 cohort studies. Cancer Epidemiol Biomarkers Prev, 21.2:305 – 318.
Gill, T. P., Rangan, A. M. and Webb K. L. (2006). The weight of evidence suggest that soft drinks are major issues in childhood and adolescent obesity. Medical journal of Australia. 184(6): 263-4.
Glinsmann, W. H., Irausquin, H., Park, Y. K. (1986). Evaluation of health aspects of sugars contained in carbohydrate sweeteners. Report of Sugars Task Force. J Nutr 116:S1–S216.
Goerss, A. L., Wagner, G. C. and Hill, W. L. (2000). Acute effects of aspartame on aggression and neuro chemistry of rats. Life Sci. 67: 1325–1329.
Gombos, K., Varjas, T., Orsós, Z., Polyák, E., Peredi, J., Varga, Z., Nowrasteh, G., Mucsi, G. and Ember, I. (2007). The effect of aspartame administration on oncogene and suppressor gene expressions. In Vivo 21: 89–92.
Gougeon, R., Spidel, M., Lee, K., and Field, C. J. (2004). Canadian diabetes association national nutrition committee technical review: Non-nutritive intense sweeteners in diabetes management. Canadian Journal of Diabetes 28: 385–399.
Graham, T. E. (2001). Caffeine and exercise: metabolism, endurance and performance. Sports Med, 31: 785–807.
Gunter, T. E., Miller, L. M., Gavin, C. E., Eliseev, R., Salter, J., Buntinas, L., Alexandrov, A., Hammond, S. Gunter, K. K. (2004). Determination of oxidation state of manganese in brain, liver, heart mitochondria. J. neurochem., 88: 266-280.
Hamilton, I. M., Gilmore, W. S., Strain, J. J., (2000). Marginal copper deficiency and atherosclerosis. Biol. Trace Elem. Res. 78: 179–189.
Hartley, T. R., Lovallo, W. R., Whitsett, T. L. (2004). Cardiovascular effects of caffeine in men and women. Am J Cardiol, 93: 1022-6.
Haschemeyer, R. H. and Haschemeyer, A. E. V. (1973). Proteins: A guide to study by physical and chemical methods vol I (New York: John Wiley and Sons) pp 1–30
Hasegawa, N. and Mori, M. (2000). Effect of powdered green tea and its caffeine content on lipogenesis and lipolysis in 3T3-L1 cell. J Health Sci 46: 153–5.
Hawkes, C. (2010). The worldwide battle against soft drinks in school. Medical journal of preventive medicine. 38(4): 457-61.
Health Canada. (2010). Caffeine. Retrieved from: http://www.hc-sc.gc.ca/hl-vs/iyh-vsv/food-aliment/caffeine-eng.php
Heckman, M. A., Weil, J., Gonzalez de Mejia, E., (2010). Caffeine (1, 3, 7-trimethylxanthine) in foods: a comprehensive review on consumption, functionality, safety, and regulatory matters. J. Food Sci. 75: R77–R87.
Hodgson, J. M., Burke, V., Puddey, I. B. (2005). Acute effects of tea on fasting and postprandial vascular function and blood pressure in humans. J Hypertens, 23: 47-54.
Homler, J. (1984). Aspartame: implications for the food scientist. In: Aspartame. Physiology and biochemistry. Eds Stegink .L D. and Filer L. J. Jr. Marcel Dekker Inc, New York, USA. 247-262.
Huxtable, R. J. (1992). Physiological actions of taurine. Physiol Rev; 72:101–63.
Imagawa, T. F., Hirano, I., Utsuki, K., Horie, M., Naka, A., Matsumoto, K., Imagawa, S. (2009). Caffeine and taurine enhance endurance performance. Int J Sports Med, 30: 485–8.
Institute of Medicine (2001). Food and Nutrition Board. Dietary Reference Intakes for Vitamin A, Vitamin K, Arsenic, Boron, Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium, and Zinc. Washington, DC: National Academy Press. Pp 7-30
Iyadurai, S. J. and Chung, S. S. (2007). New-onset seizures in adults: possible association with consumption of popular energy drinks. Epilepsy Behav. 10(3): 504–508.
Jacobson, M. (2005). Liquid candy: How soft drinks are harming American health. Center for science in the public interest. J. Health Sci. 56-58.
James, J. E. (2004). Critical review of dietary caffeine and blood pressure: A relationship that should be taken more seriously. Psychosom. Med., 66:63–71.
Jarup, L., Berglund, M. Elinder, C. Nordberg ,G. and Vahter, M. (1998). Health effects of cadmium exposure-a review of the literature and a risk estimate Scand .J.WorkEnviron.Health 24: 1-51.
Jeganathan, P. S. and Namasivayam, A. (1998). Methanol induced biogenic amine changes in discrete areas of rat brain: Role of simultaneous ethanol administration. Indian J. Physiol. Pharmacol. 32: 1–10
Jensdorttir, T., Arnadottir, B., Thordottir, I. Bardow, A. Gudmundsson, K. Theodor, A. and Holbrook, W. K. (2004). Relationship between dental erosion, soft drink consumption and gastro esophageal reflux among Icelanders. Clinical oral investigation. 8(2): 91-6.
Johnson, R. K., Appel, L. J., Brands, M., (2009). Dietary sugars intake and cardiovascular health: a scientific statement from the American Heart Association. Circulation.; 120:1011-20.
Kaminer, Y. (2010). Problematic use of energy drinks by adolescents. Child and Adolescent Psychiatric Clinics of North America. 19(3): 643-650.
Kanumakala, S., Boneh, A., and Zacharin, M., (2002). Pamidronate treatment improves bone mineral density in children with Menkes disease. J. Inherit. Metab. Dis. 25: 391–398.
Kaplan, G. B., Greenblatt, D. J., Ehrenberg, B. L., Goddard, J. E., Cotreau, M. M., Harmatz, J. S., and Shader, R. I. (1997). Dose-dependent, pharmacokinetics and psychomotor effects of caffeine in humans. Pp 608-620.
Karatzis, E, Papaioannou, T. G, and Aznaouridis, K. (2005). Acute effects of caffeine on blood pressure and wave reflections in healthy subjects: should we consider morning central blood pressure? Int J Cardiol, 98: 425-30.
Kennedy D. O., and Scholey, A. B. (2004). A glucose-caffeine ‘energy drink’ ameliorates subjective and performance deficits during prolonged cognitive demand. Appetite; 42: 331-3.
Khaw, K. T., Barrett-Connor. (1984). Dietary Potassium and Blood Pressure in a Population. Am J. Clin. Nutr. 39: 963-68
Krachler, B., Eliasson, M., Stenlund, H., Johansson, I., Hallmans, G. and Lindahl, B. (2006). Reported intake and distribution of body fat: A repeated cross section study. Nutrition Journal. 5: 34.
Krejpcio, Z., Sıonkowski, S., Bartela, J. (2005). Safety of fresh fruits and juices available on the Polish market as determined by heavy metal residues. Polish Journal of Environmental Studies, 14: 877–81.
Křižek, M., Senft, M., and Moran, J. (1997): Měď a lidske tělo. Časopis lekařů českych, 136: 698–701.
Krul, C., and Hageman, G. 1998. Analysis of urinary caffeine metabolites to assess biotransformation enzyme activities by reversed-phase highperformance liquid chromatography. J. Chromatogr B Biomed Sci. Appl., 709: 27–34.
Kruse, J. A. (1992). Methanol poisoning. Intensive Care Med. 18: 391–397
Kusumi, E., Shoji, M., Endou, S., Kishi, Y., Shibata, T., Murashige, N., Hamaki, T., Matsumura, N., Yuji, K., Yoneyama, A., Kami, M. (2006). Prevelance of anemia among healthy women in metropolitan areas in Japan. Int. J. Hematol. 84: 217-219.
Lal, G. G. (2007). Getting specific with functional beverages. Food Technol 61(12):25–31.
Larsson, S. C., Bergkvist, L., Wolk, A. (2006). Consumption of sugar and sugar-sweetened foods and the risk of pancreatic cancer in a prospective study. Am J Clin Nutr, 84:1171 – 1176.
Lee, T. K., Johnke, R. M., Allison, P. R., Obrien, K. F., Dobbs L. (2005). Radioprotective potential of ginseng. Mutagenesis, 20:237–43.
Levy, G. and Tapsell, L. (2007). Shifts in purchasing pattern 0f non alcoholic water based beverages in Australia. Nutrition diabetes. Vol (64)4.
Lima, W. P., Carnevali, L. C., Eder, R., Fernando, L., Costa Rosa, B. P., Bacch,i E. M., Seelaender, M. C. L. (2005). Lipid metabolism in trained rats: effect of guarana (Paullinia cupana Mart.) supplementation. Clin Nutr 24: 1019–28.
Lopez-Garcia, E., Van Dam, R. M., Rajpathak, S., Willett, W. C., Manson, J. E., and Hu, F. B. (2006). Changes in caffeine intake and long-term weight change in men and women. Am J Clin Nutr, 83:674–80.
Lorist, M., and Tops, M. M. (2003). Caffeine, fatigue and cognition. Brain Cogn 53: 82– 94.
Lovett R., (2005). The Demon drink: New Science. 2518.
Ludwig, D. S., Ebbeling, C. B., Peterson, K. E. and Gprtmaker, S. L. (2004). Hard facts about soft drinks. Archives and adolescent medicine. Vol 158(3): 290.
Ludwig, D. S., Peterson, K. E. and Gprtmaker, S. L. (2001). Relationship between comsumption of sugar sweetened beverages and childhood obesity: a prospective observational analysis. Lancet. Vol 357: 505-8.
Mackenzie, R. and Erik, S. (2011). Simultaneous determination of aspartame, benzoic acid, caffeine, and saccharin in sugar-free beverages using HPLC. Concordia College Journal of Analytical Chemistry I, 73-77.
Maduabuchi, J. M. U., Nzegwu, C. N., Adıgba, E. O., Aloke, R. U., Ezomike, C. N., Okocha, C. E., Obi, E., and Orisakwe, O. E. (2006). Lead and cadmium exposures from canned and non-canned beverages in Nigeria: A public health concern. Science Total of Environment, 366: 621–626.
Maduabuchi, J. M. U., Nzegwu, C. N., Adıgba, E. O., Aloke, R. U., Ezomike, C. N., Okocha C. E., Obi, E., and Orisakwe, O. E. (2008). Iron, Manganese and Nickel exposures from canned and non-canned beverages in Nigeria: A public health concern. Journal of public health Science, 54(2)335-338
MAFF (1998). Ministry of Agriculture, Fisheries and Food. Metals and other elements in beverages. Food Surveillance Information Sheet. Geneva. vol. 165.
Maher, T. J. and Wurtman, R. J. (1987). Possible neurologic effects of aspartame, a widely used food additive. Environ. Health Perspect. 75: 3–57
Malik, V. S., Schulze, M. B., and Hu, F. B. (2006). Intake of sugar sweetened beverages and weight gain: a systematic review, American Journal of Clinical Nutrition, Vol. 84(2): 274-88.
Malinauskas, B. M., Aeby, V.G., Overton, R.F., Carpenter-Aeby, T., and Barber-Heidal, K. (2007). A survey of energy drinks consumption patterns among college students. Nutritional Journal, 6: 1-7.
Marschner, H. (1995). Mineral nutrition of higher plants. Oxford University Press, London. 14: 13-20
Marshall, T. A., S. M. Levy, B. Broffit, J. J. Warren, J. M. Eichenberger-Gilmore, T. L. Burns, and Stambo, P. J. (2003). Dental carries and beverage consumption in young children. Pediatrics, 112: 184-189.
Matthews, D. M. (1984). Absorption of peptides, amino acids, and their methylated derivatives. In Aspartame: Physiology and Biochemistry (L. D. Stegink and L. J. Filer Jr., Eds.). Marcel Dekker, New York, pp. 29–46.
Maurice, S. M. (2010). The soft drinks companion: A technical handbook for the beverage industry, CRC Press. p. 45.
Meese, R. B. (1987). Epidemiologic Association between Dietary Calcium intake and Blood Pressure in Primary Hypertension. Am. J. Med.sci. 29: 4219-24
Mei, M. A., Mawahib, E., Mawahib, Mohammed, I .T., Badawi, A. Z. and Abdalla, A. E. (2012). Determination of caffeine in Some Sudanese Beverages by High Performance Liquid Chromatography. Pakistan Journal of Nutrition. 11(4): 336-342.
Miller, G. L. (1959). Use of dinitrosalicylate reagent for determination of reducing sugar. Annal Chem., 31: 427- 431.
Mohammed, S. G., Al-Hashimi, A. G. and Al-Hussainy, K. S. (2012). Determination of Caffeine and Trace Minerals Contents in Soft and Energy Drinks Available in Basrah Markets. Pakistan Journal of Nutrition 11 (9): 747-750, 2012
Mortada, W. I., Sobh, M. A., El-Defrawy, M. M., Farahat, S. E. (2001). Study of lead exposure from automobile exhaust as a risk for nephrotoxicity among traffic policemen. Am J Nephrol; 21: 274–9
Murray, R., Frankowski, B. and Taras, H. (2005). Are soft drinks a scape goat for childhood obeity? Journal of pediatrics. Vol 146(5): 586-90.
Nam, M. H., Kim, S. I., Liu, J. R., Yang, D. C., Lim, P. Y., Kwon, K. H., Yoo, J. S., Park, Y. M. (2005). Proteomic analysis of Korean ginseng (Panax ginseng C.A. Meyer). J Chromatogr; 815: 147–55.
Nash, J. (1992). “Health Contenders.” Essence. 23: 79-81.
Nawrot, P., Jordan, S., Eastwood, J., Rotstein, J., Hugenholtz, A., Feeley. M. (2003).Effects of caffeine on human health. Food Addit Contam; 20(1):1-30.
Nestle, M. (2005). Preventing childhood diabetics: The need for public health intervention. American journal of public health. 95(9): 1497-9.
Newsome, R. L. (1986). Sweeteners: Nutritive and non-nutritive; in The Scientific status summaries of the Institute of Food Technologies Expert Panel on Food Safety and Nutrition (Chicago: Institutive of Food Technologies). Pp 180.
Norbiato, G., Bevilacqua M., Merino R. (1984). Effects of Potassium supplementation on Insulin Binding Action in Human Obesity: Protein Modified fast and Feeding. Europ J. Clin. Invest. 44: 414-19.
Obuzor, G. U. and Ajaezi, N. E. (2010). Nutritional content of popular malt drinks produced in Nigeria. African Journal of Food Science Vol. 4(9), pp. 585 – 590.
O’Dea J. A. (2003). Consumption of nutritional supplements among adolescents: usage and perceived benefits. Health Education Research; 18: 98-107.
Olney, J. W., Labruyere, J. and De Gubareff, T. (1980). Brain damage in mice from voluntary ingestion of glutamate and aspartate. Neurobehav. Toxicol. 2: 125–129.
Onianwa, P. C., Adeyemo, A. O., Idowu, E. O., Ogabiela, E. E. (2001). Copper and Zinc Content of Nigeria Food and Etsimate of the Adult Dictionary intakes. Food Chem. 72: 89 – 95.
Onianwa, P. C., Adetol, I. G., Iwebue, C. M. A., Oto, M. F., Tella, O. O. (1999). Trace heavy metals composition of some Nigerian beverages and food drinks. Food Chemistry,66: 275-279.
Oppermann, J. A., Muldoon, E., and Ranney, R. E. (1973). Metabolism of aspartame in monkeys. Journal of Nutrition 103: 1454–1459.
Pan-Hou H., Suda Y., Ohe Y., Sumi, M. and Yoshioka, M. (1990). Effect of aspartame on N-methyl-D-aspartate-sensitive L-[3H]glutamate binding sites in rat brain synaptic membranes. Brain Res. 520: 351–353
Penland, J. G., Johnson, P. E. (1993). Dietary Calcium and Manganese, Effects on Menstrual Cycle Symptoms. Am Journ Obstet Gynecol. 168: 1417-23.
Pennay, A., Lubman, I. D., and Miller, P. (2011). Combining Energy Drinks and Alcohol.
Australian Family Physician. 40: 104-105
Pettenuzzo, L. F., Noschang, C., Von Pozzer Toigo, E., Fachin, A., Vendite, D., Dalmaz, C. (2008). Effects of chronic administration of caffeine and stress on feeding behavior of rats. Physiol Behav. 95: 295–301.
Prankerd, R. J. (2002). Aspartame. Analytical Profiles of Drug Substances and Excipients, 29: 7-55.
Prasad, A. S. (1995). Zinc: an overview. Nutrition; 11: 93-99.
Rabin, O., Hegedus, L., Bourre, J. M., Smith, Q. R., (1993). Rapid brain uptake of manganese(II) across the blood–brain barrier. J. Neurochem. 61: 509–517.
Rachel, R. M., Bruce, A. G., Edward, J. C. (2006). Caffeine Content of Energy Drinks, Carbonared Sodas and other Beverages. Journal of analytical toxicology. Vol. 30
Rangan, A. M., Randall, D., Hector, D. J., Gill, T. P. And Webb, K. L. (2008). Consumption of extra food by Australian children: types, quantities and contribution to energy and nutrients intake. European Journal of clinical Nutrition. 62(3): 356-64
Ranney, R. E., and Oppermann, J. A. (1979). A review of the metabolism of the aspartyl moiety of aspartame in experimental animals and man. Journal of Environmental Pathology and Toxicology 2: 979– 985.
Rath, M. (2012). Energy drinks: What is all the hype? The dangers of energy drink consumption. Journal Of The American Academy Of Nurse Practitioners, 24(2): 70-76.
Reid, I. R. (1995). Long Term Effects of Calcium Supplementation on Bone Loss and fractures I Post-Menopausal Women. A Randomized Control Trial. Am. J. Med Sci. 98: 331-35.
Reissig, C. J., Strain, E. C., Griffiths, R. R. (2008). Caffeinated energy drinks – A growingproblem. Drug and Alcohol Dependence. 99: 1-10.
Reyner, L. A., and Horne, J. A. (2002). Efficacy of a ‘functional energy drink’ in counteracting driver sleepiness. Physiol Behav.; 75: 331-5.
Riesenhuber, A, Boehm, M, Posch, M, Aufricht, C. (2006). Diuretic potential of energy drinks. Amino Acids, 31: 81–3.
Rubio, C., Hardisson, A., Reguera, J. I., Revert, C., Lafuente ,M. A., Gonzalez-Iglesias T. (2006). Cadmium dietary intake in the Canary Islands, Spain. Environmental Research, 100: 123–129.
Ryan-Harshman, M., Aldoori, W. (2005). Health benefits of selected minerals, Can. Fam Physician. 51(5): 673–675.
Satarug, S., and Moore, M. R. (2004). Adverse health effects of chronic exposure to low-level cadmium in foodstuffs and cigarette smoke. Environ. Health Prespect. 112(10): 1099.
Savoca, M. R., Evans, C. D., Wilson, M. E., Harshfield, G. A., Ludwig, D. A. (2004). The association of caffeinated beverages with blood pressure in adolescents. Arch Pediatr Adolesc Med.; 158: 473-7.
Schernhammer, E. S., Hu, F. B., Giovannuci, E., Michaud, D. S., Colditdz, G. A., Stampfer, M. J. and Fuchus, C. S. (2005). Sugar sweetened soft drinks consumption and risk of pancreatic cancer in two perspectives, Cancer epidemiology, Biomarkers and Prevention. 14(7): 2098-105.
Scholey, A. B., and Kennedy, D. O. (2004). Cognitive and physiological effects of an “energy drink”: an evaluation of the whole drink and of glucose, caffeine and herbal flavouring fractions. Psychopharmacology (Berl); 176: 320-30.
Schulze, M. B., Manson, J. E., Ludwig, D. S., Colditz, G. A., Stampfer, M. J., Willett, W. C., Hu, F.B. (2004). Sugar-sweetened beverages, weight gain, and incidence of type 2 diabetes in young and middle-aged women. JAMA, 292(8): 927 – 934.
Seidal, K., Jorgensen, N., Elinder, C. G., Sjogren, B., Vahter, M. (1993). Fatal cadmium-induced pneumonitis. Scand J Work Environ Health; 19: 429–31
Senesse. P., Meance, S., Cottet, V., Faivre, J., Boutron-Ruault, M. C. (2004). High dietary iron and copper and risk of colorectal cancer: a case-control study in Burgundy, France. Nutr. Cancer. 49: 66-71
Serdar, M. and Knežević, Z. (2011). Hplc determination of artifical sweeteners in beverages and special nutritional Products using high performance liquid Chromatography. Arh Hig Rada Toksikol; 62: 169-173.
Shapiro. R. B. (1988). Statement for the labor and human resources committee, US Senate (Washington, DC, Government Printing Office). 12: 5-7
Shenkin, J. D., Heller, K.E., Warren, J. J., and Marshal, T. A. (2003). Soft drink consumption and carries in children and adolescents. General dentistry. 51(1): 30-6.
Siegel R. K. (1979). Ginseng abuse syndrome. Problems with the panacea. JAMA; 241: 1614-5.
Smith, H. J. and Rogers, P. J. (2002). Effects of energy drinks on mood and mental performance: critical methodology. Food Qual Pref, 13: 317–26.
Sower, J. R. (1989). Calcium and Hypertention. Journal Lab Clin. Med. 114: 338-48Stegink, L. D., Brummel, M. C., Filer, L. J. and Baker, G. L. (1983). Blood methanol concentrations in one year old infants administered graded doses of aspartame. J. Nutr. 113 1600–1606.
Spiller, M. A. (1998). The Chemical Components of Coffee. In: Caffeine. Spiller, G. A., Ed., CRC Press, Boca Raton. 97–161.
Stegink, L. D. (1987). The aspartame story: A model for the clinical testing of a food additive. American Journal of Clinical Nutrition 46: 204–215.
Stipanuk, M. H. (2004). Role of liver in the regulation of body cysteine and taurine levels: a brief review. Neurochem Res, 29:105–10.
Striegel-Moore, R. H, Thompson, D., Affenitto, S. G., Franko, D. L., Obarzanek, E., Barton, B. A., Daniels, S. R., Schmidt, M. and Crawford, P. B. (2006). Correlates of beverage intake in adolescent girls: The National Heart, Lung, and Blood Institute Growth and Health Study, Journal of pediatrics. Vol. 148: 183-7.
Takagi, I. (1991). Calcium Treatment of Essential Hypertension in Elderly Patients Evaluated by 24H Monitoring. Am J. Hypertens. 4: 836-9
Thys-Jocos, S. (1989). Calcium Supplementation in Pre-Menstrual Syndrome. A Randomized Cross Over trial. Gen. Intern. Med. 4: 183-9
Timbrell J. A., Seabra V., Waterfield C. J. (1995). The in vivo and in vitro protective properties of taurine. Gen Pharmac. 26:453–62.
Tremblay, A., Masson, E., Leduc, S., Houde, A., Despres, J. P. (1988). Caffeine reduces spontaneous energy intake in men but not in women. Nutr Res 8: 553–8.
(USEPA) (United State Environmental Protection Agency) (1985). Consumer fact sheet. Protection Agency, Environmental Criteria and Assessment Office. Pp 10-20.
(USFDA) (2010). (United State Food and Drug Administration Overview of dietary supplements. Available at:www.fda.gov/Food/DietarySupplements/ConsumerInformation/ucm110417.htm Accessed January 17, 2011
USDA/HHS, (U.S. Department of Agriculture/U.S. Department of Health and Human Services). 2000. Nutrition and Your Health: Dietary Guidelines for Americans. Washington, DC: U.S. Government Printing Office. Home and Garden Bulletin No. 232.
Van den Eynde, F., Van Baelen, P. C., Portzky, M. Audenaert, K. (2008). The effects of energy drinks on cognitive performance.Psychiatric, 50(5): 273-281.
Vartanian, L. R., Schwartz, M.B., Brownell, K.D. (2007). Effects of soft drink consumption on nutrition and health: A systematic review and meta-analysis. Am J Public Health, 97, 667 – 675.
Wardlaw, G. M. and Smith, A. M. (2009). Contemporary nutrition a functional approach. New York: McGraw-Hill. Pp 18-22.
WHO, (World Health Organization) (1995). Lead: Environmental Health Criteria,: World Health Organization, Geneva. vol. 165.
WHO, (World Health Organization) (1993). Micronutrient deficiency. Battling iron deficiency anemia [online] Nutritional program. Geneva. vol. 16.
Yokogoshi, H., Roberts, C. H., Caballero, B. and Wurtman, R. J. (1984). Effects of aspartame and glucose administration on brain and plasma levels of large neutral amino acids and brain 5-hydroxyindoles. Am. J. Clin. Nutr. 40: 1–7.
Zheng, G., Sayama, K., Okubo, T., Juneja, L. R., Oguni, I. (2004). Anti-obesity effects of three major components of green tea, catechins, caffeine and theanine, in mice. In Vivo, 18: 55–62.
Żółtaszek, R., Hanausek, M., Kilian´ska, Z. M., Walaszek, Z. (2008). The biological role of D-glucaric acid and its derivatives: potential use in medicine [in Polish]. Postepy Hig Med Dosw (Online). 62: 451-462.