ISOLATION AND CHARACTERIZATION OF BACTERIA ASSOCIATED WITH HAWKED SUYA-MEAT

ABSTRACT

This project work is carried at the isolation and characterization of pathogenic bacteria from hawked suya meat, were bought in full from the sellers and was collected in raps of aluminum foil. It was then taken to the laboratory for analysis the samples were homogenized and serial dilution of the sample was made out using 5 ml sterile pipette and test tubes. The dilution were then cultured on different media, macconkey agar, nutrient agar and blood agar end later incubated at 370c for 24 hours. Discrete bacteria colonies were observed and each colomy was gram stained and examined microscopically. Biochemical test were carried out to confirm the particular bacteria present. Bactering isolated were Staphylococcus aureus, Bacillus, lactobacillus, streptococcus and Escherichia coli. Most at this microorganisms isolated are pathogenic and are toxic when ingested in contaminated suya meat. They are capable of causing illness. For instance staphylococcus aureus produce air exotizcin and can with stand temperature of 1000c for 30 minutes. When ingested, if cause intoxication manifesting in 4-5 hours with acute diarrhoes vomiting and gastroertrities lasting 6-8 hours.

TABLE OF CONTENT

Title page

Certification

Dedication

Acknowledgement

Abstract

Table of contents

CHAPTER ONE

INTRODUCTION

Aims and objective

Hypothesis

Statement of problem

Significance

Limitation

CHAPTER TWO

Literature review

CHAPTER THREE

Materials

Reagents

Media used

Sugar used for biochemical test

Preparation of media

Collection of samples

Method used

Pour plate method

Staining technique used

Biochemical test used

Coagulates test

Motility test

Indole test

Sugar fermentation test

CHAPTER FOUR

Result of the analysis

Identification of the isolate

Confirmatory result

CHAPTER FIVE

Discussion and conclusion

Recommendation

References