ISOLATION AND IDENTIFICATION OF BACTERIA ASSOCIATED WITH WOUND SEPSIS

ABSTRACT

The isolation and identification of bacteria associated with 150ml sepsis studies were carried out to 250 patient at National Orthopaedic Hospital Enugu. (250) Two hundred and fifty patients with 150ml sepsis was grouped in to in patients and out patients. The sample collected are wound pus and wound exudates which were collected from patients with diabetic ulcer, surgery wounds, sickle cell ulcers, gun shot wound, using sterile stent striates to aspirate the wound exudates and stet swab sticks to swab the wound prof. Population was sampled

According to wound type and noting their differences sex distributing palternage range (0-2_ years, (3-5_ years (608) years (9-11_ years, (12-14) years, (15-17) years, (18-20) years, and 21 years and above, in sex distribution. (male and female), in predisposing factors and in any other factor affecting it and also their antibiotic sensitivity pattern. The samples collected were plated out to on Mac conkey agar, Blood agar, Perory citrate agar (DCA) and further examination was done such as motility test gram staining and some brolhemoceltest like citrate, on and catalyst test for confirmation. The results obtained from 250 patients samples, 199 were positive pathogens and 51 were negative out of which 97 (48.890) were staphylococcus aureus, Staphylococcus aureus was recorded as the organism that was the highest prevelence while galmorella species has the lowest prevalence 3(1.5%) In this study. The sensivity patttern of the isolated organisms results show that pseudomonas aeruginosa is resistant to more drugs than the staphylococcus aureus and escherichris coli. Most organisms were sensitive to Ciprofloxacin, Ampicillin, Floxacillin, Erythromycin, Cotrimoxazole.